Pleural Effusions

The second study is called "fat cell membranes revealed immunocytochemical staining: a trace of a diagnosis of mesothelioma," Anthony Y. Leong MD, MB, FRCP, FRCPath, FCAP, Richard Parkinson, James Milios HT. - Diagnostic Cytopathology - Volume 6, Number 1, pages 9-13, January 1990 Here's an excerpt: "
Summary - a difference of malignant mesothelioma from metastatic adenocarcinoma in pleural effusions and biopsies are often diagnostic problem. Immunocytochemical staining of 13 malignant mesotheliomas, eight of primary adenocarcinoma of the lung, five metastatic adenocarcinoma of the lung, and 20 primary adenocarcinomas of the extrapulmonary sites with a monoclonal antibody to epithelial membrane antigen (EMA) revealed a 'thick' of the cell membrane in all cases of mesothelioma. This characteristic pattern of staining was seen on the periphery of cell clusters and circumferentially around individual cells in cytologic preparations, cell blocks and tissue sections. Intracel-Mar and the intercellular acini are listed by the anti-EMA, and intraluminal long microvillous projections showed. Weak cytoplasmic staining is only rarely seen in mesothelioma cells. This membranous staining pattern was not observed in adenocarcinomas, which shows strong and diffuse cytoplasmic bojenje.Imunocitokemijska demonstration of dense and spiky membrane circumferentially disposed around individual cells corresponding to a distorted microvilli, a diagnostic clue in identifying malignant mesothelioma ."

Summary - a difference of malignant mesothelioma from metastatic adenocarcinoma in pleural effusions and biopsies are often diagnostic problem. Immunocytochemical staining of 13 malignant mesotheliomas, eight of primary adenocarcinoma of the lung, five metastatic adenocarcinoma of the lung, and 20 primary adenocarcinomas of the extrapulmonary sites with a monoclonal antibody to epithelial membrane antigen (EMA) revealed a 'thick' of the cell membrane in all cases of mesothelioma. This characteristic pattern of staining was seen on the periphery of cell clusters and circumferentially around individual cells in cytologic preparations, cell blocks and tissue sections. Intracel-Mar and the intercellular acini are listed by the anti-EMA, and intraluminal long microvillous projections showed. Weak cytoplasmic staining is only rarely seen in mesothelioma cells. This membranous staining pattern was not observed in adenocarcinomas, which shows strong and diffuse cytoplasmic bojenje.Imunocitokemijska demonstration of dense and spiky membrane circumferentially disposed around individual cells corresponding to a distorted microvilli, a diagnostic clue in identifying malignant mesothelioma ."